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ATCC s mitis atcc 49456
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ATCC atcc 49455t
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ATCC streptococcus sanguinis
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ATCC streptococcus mitis atcc 49456
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49455t  (ATCC)
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ATCC phosphoglucomutase gene
Bacterial strains and primers used in the study
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ATCC actinomadura fibrosa atcc 49459t
Bacterial strains and primers used in the study
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ATCC oral streptococci s mitis atcc 49456
Bacterial strains and primers used in the study
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ATCC oral pathogens
Bacterial strains and primers used in the study
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Bacterial strains and primers used in the study
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ATCC strains s pseudopneumoniae atcc baa 960t
Bacterial strains and primers used in the study
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r001t  (ATCC)
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Bacterial strains and primers used in the study
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Bacterial strains and primers used in the study

Journal: Journal of Bacteriology

Article Title: Effect of Periodontal Pathogens on the Metatranscriptome of a Healthy Multispecies Biofilm Model

doi: 10.1128/JB.06328-11

Figure Lengend Snippet: Bacterial strains and primers used in the study

Article Snippet: DNA was extracted as described above. table ft1 table-wrap mode="anchored" t5 caption a7 Bacterial strain or RT-qPCR primer Forward primer 5′–3′ Reverse primer 5′–3′ Target gene (source or reference) Bacterial strains Actinomyces naeslundii (MG1) GAGAAGAACTCCCTATCCATACC CTTCATGGGTTGGGATTTCCT Target is fimA gene, a fimbrial protein (this work) Fusobacterium nucleatum (ATCC10953) CTTATACATAGAGGACACAGAC TTTCCAACAACATCTCCCTG HprK gene ( 30 ) homologue of HPr kinase/phosphorylase (this work) Lactobacillus casei (ATCC 334) TGAATATTTACACGGTTCGGCA GGGCCTTAGTTTCATCCGAC Target is the phosphoglucomutase gene ( 10 ) (this work) Streptococcus mitis (NCTC 12261, ATCC 49456) CGTATCGGTCGTCTTGCT TTGCATAACTGGATCTGTAAGGTC Target is the glyceraldehyde-3-phosphate gene (this work) Veillonella parvula (ATCC17745) ACAGTTACTTTATAGTCTGTACC TGGTCAATTGGCTAAACGTCAA Target is the chaperone dnaK gene ( 29 ) (this work) Aggregatibacter actinomycetemcomitans (HK1651) ACGCAGACGATTGACTGAATTTAA GATCTTCACAGCTATATGGCAGCTA Target is lktC gene part of the leukotoxin operon lktBAC ( 54 ) Porphyromonas gingivalis (ATCC 33277) CCTACGTGTACGGACAGAGCTATA AGGATCGCTCAGCGTAGCATT Target is the Arg-gingipain gene ( 54 ) RT-qPCR primers Universal CGCTAGTAATCGTGGATCAGAATG TGTGACGGGCGGTGTGTA 16S rRNA ( 79 ) Actinomyces naeslundii (MG1) ATCTCCAAGGTTCTGCACGACGAGTA ATGTTGATGGTGATACCGCGCTGA ANA_0022 (this work) Aggregatibacter actinomycetemcomitans (HK1651) AAGAACTTAACGCTTGGGCGATGC TAACGCTTCTTGTGCAACACTGGC AA00470 (this work) Veillonella parvula (ATCC17745) AAAGCCTTGGGCCATTCTCTGTTG CCAAGGCCTCTTGTTCTTGCATCA HMPREF1035_1004 (this work) Porphyromonas gingivalis (ATCC 33277) AGAAAGCCAGCCATTGTTGCATGG TGTTCGGGACACCTGACTGTTCAT PGN_0074 (this work) Open in a separate window Bacterial strains and primers used in the study Doubling time was calculated using the following formula: t d = ( t 2 − t 1 ) × log (2)/log( q 2 / q 1 ), where q 2 represents the final number of cells, q 1 represents the initial number of cells added, and ( t 2 − t 1 ) represents the interval of incubation.

Techniques: